1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94
//! Create random reads from stdin.
//!
//! # Examples
//!
//! ```perl
//! print "hello world\n";
//! ```
//!
//! ## General usage
//! General usage to randomize the order of the reads
//! ```bash
//! cat file.fastq | fasten_randomize > random.fastq
//! ```
//! ## One read
//! Get one random read. Entries will always be in a 4-line format.
//! ```bash
//! cat file.fastq | fasten_randomize | head -n 4 > one_read.fastq
//! ```
//! ## Paired end
//! If paired, keep them together
//! ```bash
//! cat R1.fastq R2.fastq | fasten_shuffle | fasten_randomize --paired-end | head -n 8 > one_pair.fastq
//! ```
//!
//! # Usage
//!
//! ```text
//! Usage: fasten_randomize [-h] [-n INT] [-p] [-v]
//!
//! Options:
//! -h, --help Print this help menu.
//! -n, --numcpus INT Number of CPUs (default: 1)
//! -p, --paired-end The input reads are interleaved paired-end
//! -v, --verbose Print more status messages
//! ```
extern crate getopts;
extern crate fasten;
extern crate rand;
use std::fs::File;
use std::io::BufReader;
use std::io::BufRead;
use rand::seq::SliceRandom;
use fasten::fasten_base_options;
use fasten::fasten_base_options_matches;
fn main(){
let opts = fasten_base_options();
let matches = fasten_base_options_matches("Create random reads from stdin.", opts);
let lines_per_read :u32={
if matches.opt_present("paired-end") {
8
}else{
4
}
};
print_reads_from_stdin(lines_per_read);
}
/// Read fastq from stdin, add the reads to a vector,
/// then print them to stdout in random order
fn print_reads_from_stdin(lines_per_read :u32) -> () {
// Start off with a capacity of 100k reads.
let mut seqs :Vec<String> = Vec::with_capacity(100000);
let my_file = File::open("/dev/stdin").expect("Could not open stdin");
let my_buffer=BufReader::new(my_file);
let mut lines = my_buffer.lines();
while let Some(id) = lines.next() {
let mut entry = id.expect("ERROR: could not parse the ID line");
for _ in 1..lines_per_read {
entry.push('\n');
let next_line = lines.next()
.expect("ERROR: could not get the next line")
.expect("ERROR: could not parse the next line");
entry.push_str(&next_line);
}
seqs.push(entry);
}
// choose random reads
let mut rng = rand::thread_rng();
//rng.shuffle(&mut seqs);
seqs.shuffle(&mut rng);
for seq in seqs {
println!("{}",seq);
}
}